mab anti cd55 Search Results


anti-cd55 monoclonal antibody mab gtx113170  (GeneTex)
90
GeneTex anti-cd55 monoclonal antibody mab gtx113170
Anti Cd55 Monoclonal Antibody Mab Gtx113170, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd55 monoclonal antibody mab gtx113170/product/GeneTex
Average 90 stars, based on 1 article reviews
anti-cd55 monoclonal antibody mab gtx113170 - by Bioz Stars, 2026-05
90/100 stars
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anti-hdaf mouse mab anti-cd55  (Merck KGaA)
90
Merck KGaA anti-hdaf mouse mab anti-cd55
Schematic representation of BDES constructs and the expression of functional proteins. a <t>The</t> <t>pfcsp</t> genes were cloned from P. falciparum (PfCSP 19–373 ; PfCSP1) or synthesized with codon-optimization (synthesized PfCSP 19–377 ; sPfCSP2). Both pfcsp genes were fused to the N-terminus of the gp64 gene (gp64 21–512 ) and the transmembrane region of the vsv - g gene (VSV-G 421–511 ; G). Expression of the PfCSP gene cassette was driven by a dual promoter (pCMV and pPolh). In the “Spider” type of BDES construct, but not in the “Spier” type construct, the human daf gene <t>(hDAF)</t> was displayed on virions under the control of the p10 promoter. b AcNPV-WT (non-recombinant control), BES-GL3-Spider (hDAF-displayed control), BDES-PfCSP1-gp64, BDES-sPfCSP2-Spier, and BDES-sPfCSP2-Spider (lanes 1–5, respectively) were lysed and subjected to the immunoblots with anti-PfCSP, anti-hDAF, or anti-VP39 Abs. ( c – j ) The morphologies of BDES-sPfCSP2-Spider ( c – f ) and BDES-sPfCSP2-Spier ( g – j ) are shown by transmission electron microscopy. The viral particles were reacted with a nonspecific mouse IgG ( c , g ), anti-FLAG mAb ( d , h ), anti-PfCSP mAb ( e , i ), or anti-DAF mouse mAb ( f , j ), and then incubated with a 5 nm colloidal gold-conjugated secondary Ab. Bars, 100 nm; Arrows, colloidal gold signals
Anti Hdaf Mouse Mab Anti Cd55, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hdaf mouse mab anti-cd55/product/Merck KGaA
Average 90 stars, based on 1 article reviews
anti-hdaf mouse mab anti-cd55 - by Bioz Stars, 2026-05
90/100 stars
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anti-cd55 mab  (Viragen Inc)
90
Viragen Inc anti-cd55 mab
Schematic representation of BDES constructs and the expression of functional proteins. a <t>The</t> <t>pfcsp</t> genes were cloned from P. falciparum (PfCSP 19–373 ; PfCSP1) or synthesized with codon-optimization (synthesized PfCSP 19–377 ; sPfCSP2). Both pfcsp genes were fused to the N-terminus of the gp64 gene (gp64 21–512 ) and the transmembrane region of the vsv - g gene (VSV-G 421–511 ; G). Expression of the PfCSP gene cassette was driven by a dual promoter (pCMV and pPolh). In the “Spider” type of BDES construct, but not in the “Spier” type construct, the human daf gene <t>(hDAF)</t> was displayed on virions under the control of the p10 promoter. b AcNPV-WT (non-recombinant control), BES-GL3-Spider (hDAF-displayed control), BDES-PfCSP1-gp64, BDES-sPfCSP2-Spier, and BDES-sPfCSP2-Spider (lanes 1–5, respectively) were lysed and subjected to the immunoblots with anti-PfCSP, anti-hDAF, or anti-VP39 Abs. ( c – j ) The morphologies of BDES-sPfCSP2-Spider ( c – f ) and BDES-sPfCSP2-Spier ( g – j ) are shown by transmission electron microscopy. The viral particles were reacted with a nonspecific mouse IgG ( c , g ), anti-FLAG mAb ( d , h ), anti-PfCSP mAb ( e , i ), or anti-DAF mouse mAb ( f , j ), and then incubated with a 5 nm colloidal gold-conjugated secondary Ab. Bars, 100 nm; Arrows, colloidal gold signals
Anti Cd55 Mab, supplied by Viragen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd55 mab/product/Viragen Inc
Average 90 stars, based on 1 article reviews
anti-cd55 mab - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti-cd55 mab  (CancerTools Org)
N/A
CD97 is a member of the epidermal growth factor-seven transmembrane family. It affects tumour aggressiveness by binding its cellular ligand CD55 and exhibits adhesive properties. Previous studies have shown that CD97 and CD55 are involved
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Schematic representation of BDES constructs and the expression of functional proteins. a The pfcsp genes were cloned from P. falciparum (PfCSP 19–373 ; PfCSP1) or synthesized with codon-optimization (synthesized PfCSP 19–377 ; sPfCSP2). Both pfcsp genes were fused to the N-terminus of the gp64 gene (gp64 21–512 ) and the transmembrane region of the vsv - g gene (VSV-G 421–511 ; G). Expression of the PfCSP gene cassette was driven by a dual promoter (pCMV and pPolh). In the “Spider” type of BDES construct, but not in the “Spier” type construct, the human daf gene (hDAF) was displayed on virions under the control of the p10 promoter. b AcNPV-WT (non-recombinant control), BES-GL3-Spider (hDAF-displayed control), BDES-PfCSP1-gp64, BDES-sPfCSP2-Spier, and BDES-sPfCSP2-Spider (lanes 1–5, respectively) were lysed and subjected to the immunoblots with anti-PfCSP, anti-hDAF, or anti-VP39 Abs. ( c – j ) The morphologies of BDES-sPfCSP2-Spider ( c – f ) and BDES-sPfCSP2-Spier ( g – j ) are shown by transmission electron microscopy. The viral particles were reacted with a nonspecific mouse IgG ( c , g ), anti-FLAG mAb ( d , h ), anti-PfCSP mAb ( e , i ), or anti-DAF mouse mAb ( f , j ), and then incubated with a 5 nm colloidal gold-conjugated secondary Ab. Bars, 100 nm; Arrows, colloidal gold signals

Journal: Malaria Journal

Article Title: DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice

doi: 10.1186/s12936-017-2039-x

Figure Lengend Snippet: Schematic representation of BDES constructs and the expression of functional proteins. a The pfcsp genes were cloned from P. falciparum (PfCSP 19–373 ; PfCSP1) or synthesized with codon-optimization (synthesized PfCSP 19–377 ; sPfCSP2). Both pfcsp genes were fused to the N-terminus of the gp64 gene (gp64 21–512 ) and the transmembrane region of the vsv - g gene (VSV-G 421–511 ; G). Expression of the PfCSP gene cassette was driven by a dual promoter (pCMV and pPolh). In the “Spider” type of BDES construct, but not in the “Spier” type construct, the human daf gene (hDAF) was displayed on virions under the control of the p10 promoter. b AcNPV-WT (non-recombinant control), BES-GL3-Spider (hDAF-displayed control), BDES-PfCSP1-gp64, BDES-sPfCSP2-Spier, and BDES-sPfCSP2-Spider (lanes 1–5, respectively) were lysed and subjected to the immunoblots with anti-PfCSP, anti-hDAF, or anti-VP39 Abs. ( c – j ) The morphologies of BDES-sPfCSP2-Spider ( c – f ) and BDES-sPfCSP2-Spier ( g – j ) are shown by transmission electron microscopy. The viral particles were reacted with a nonspecific mouse IgG ( c , g ), anti-FLAG mAb ( d , h ), anti-PfCSP mAb ( e , i ), or anti-DAF mouse mAb ( f , j ), and then incubated with a 5 nm colloidal gold-conjugated secondary Ab. Bars, 100 nm; Arrows, colloidal gold signals

Article Snippet: The lysates were separated by 8% SDS-PAGE and transferred to polyvinylidene fluoride membranes, and then probed with either of the following: an anti-PfCSP mouse mAb (2A10) or an anti-hDAF mouse mAb (anti-CD55, Merck Millipore, Temecula, CA), together with an anti-VP39 rabbit Ab [ ].

Techniques: Construct, Expressing, Functional Assay, Clone Assay, Synthesized, Control, Recombinant, Western Blot, Transmission Assay, Electron Microscopy, Incubation

Schematic representation of the luciferase-expressing vaccines and their complement resistance in vitro and in vivo. a BES-GL3-Spier and BES-GL3-Spider vectors express firefly luciferase GL3 under the pCMV single promoter, and the BES-GL3-Spider vector contains a gene cassette for hDAF display. PB, PiggyBac transposon sequence; S, gp64 signal sequence; F, FLAG epitope tag; M, myc epitope tag; and EGFP, enhanced green fluorescent protein. b BES-GL3-Spier (lane 1) and BES-GL3-Spider (lane 2) were lysed and subjected to immunoblotting with anti-DAF and anti-VP39 Abs. c BES-GL3-Spier and BES-GL3-Spider were treated with heat-inactivated or intact serum and then transduced into HepG2 cells. Cell lysates were subjected to luciferase assays. Each relative luciferase unit (RLU) was normalized against each of the values of the heat-inactivated samples (n = 3). Bars and error bars indicate the mean ± SD of the values, respectively. Representative data from three independent studies are shown. HI, heat-inactivated serum; Intact, non-heat-inactivated serum. d Luciferase expression in BES-GL3-Spier- and BES-GL3-Spider-immunized Balb/c mice at 1, 3, 5, 7, and 14 days post-immunization (n = 4). The heat map image visible in the mice represents the total flux of photons (p/sec/cm 2 ) in that area. e The mean total flux of photons is shown. Bars and error bars indicate the mean ± SD of the values, respectively. Comparison between groups was assessed by the Mann–Whitney U -test. * p < 0.05, compared with BES-GL3-Spier

Journal: Malaria Journal

Article Title: DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice

doi: 10.1186/s12936-017-2039-x

Figure Lengend Snippet: Schematic representation of the luciferase-expressing vaccines and their complement resistance in vitro and in vivo. a BES-GL3-Spier and BES-GL3-Spider vectors express firefly luciferase GL3 under the pCMV single promoter, and the BES-GL3-Spider vector contains a gene cassette for hDAF display. PB, PiggyBac transposon sequence; S, gp64 signal sequence; F, FLAG epitope tag; M, myc epitope tag; and EGFP, enhanced green fluorescent protein. b BES-GL3-Spier (lane 1) and BES-GL3-Spider (lane 2) were lysed and subjected to immunoblotting with anti-DAF and anti-VP39 Abs. c BES-GL3-Spier and BES-GL3-Spider were treated with heat-inactivated or intact serum and then transduced into HepG2 cells. Cell lysates were subjected to luciferase assays. Each relative luciferase unit (RLU) was normalized against each of the values of the heat-inactivated samples (n = 3). Bars and error bars indicate the mean ± SD of the values, respectively. Representative data from three independent studies are shown. HI, heat-inactivated serum; Intact, non-heat-inactivated serum. d Luciferase expression in BES-GL3-Spier- and BES-GL3-Spider-immunized Balb/c mice at 1, 3, 5, 7, and 14 days post-immunization (n = 4). The heat map image visible in the mice represents the total flux of photons (p/sec/cm 2 ) in that area. e The mean total flux of photons is shown. Bars and error bars indicate the mean ± SD of the values, respectively. Comparison between groups was assessed by the Mann–Whitney U -test. * p < 0.05, compared with BES-GL3-Spier

Article Snippet: The lysates were separated by 8% SDS-PAGE and transferred to polyvinylidene fluoride membranes, and then probed with either of the following: an anti-PfCSP mouse mAb (2A10) or an anti-hDAF mouse mAb (anti-CD55, Merck Millipore, Temecula, CA), together with an anti-VP39 rabbit Ab [ ].

Techniques: Luciferase, Expressing, Vaccines, In Vitro, In Vivo, Plasmid Preparation, Sequencing, FLAG-tag, Western Blot, Comparison, MANN-WHITNEY